Oomycota

See web page: http://www.ucmp.berkeley.edu/chromista/oomycota.html

 

Characterisitics (see table for orders)

 

Zoospore: biflagellate with one flagellum tinsel and the other whiplash

Coenocytic hyphae

Cell walls composed primarily of B-glucans, hydroxyproline, and small amount of cellulose, rarely chitin (in Leptomitales , Achyla, and Saprolegnia)

Mitochrondia with tubular cristae

Golgi bodies with flattened cristate

No sporocarp

Ooogamous, Oospores (one or many), Gametic meiosis

No true male gametes

Gametangial copulation

DAP lysine synthesis

Zoosporangia or vesicles

Diplanetic zoospores

Biochemistry

Synthesis of thiamine

Utilization of nitrates and sulfates

Can synthesize sterols: fuscosterol

Storage compound: mycolaminarin

Absence of sugar alcohols

 

Orders

Saprolegniales

Peronosporales

Leptomitales

Lagenidiales

Primary zoospores

+

-

in some

+

Secondary zoospores (see fig. 27.2, 27.5 in handout)

+; dimorphic

+; monomorphic in Pythium, Phytophthora.

Mostly; about morphic

+? About morphic

Sporangia differentiation

none

well

similar to saprolegniales

well

Vesicles see fig. 27.4 in handout)

no

in some (Pythium; Phytopthora; not in Albugo

no

present

#oospheres/oogonium

several

1

1

1

synthesis of thiamine

+

-

+

?

utilize nitrates

-

+

-

?

utilize sulfates

-

+

+

-

special features

variation in genera where secondary zoospores are produced; sporangial proliferation; gemmae

mostly obligate parasites of animals or plants (downy mildews, white blister rust, potato blight)

contrction, plugged with cellulin (mixture of chitin and glucan; function?); Aqualiderella (high co2, low to no O2; on debris in clear water or in stagnant ponds

holocarpic, endobiotic, parasitic in algae and roots of vascular plants.

 

Saprolegniales

Where secondary zoospores produced: Saprolegnia (outside of sporangia); Achlya (at tip of sporangium, kept together by hooks); Dictyuchus (in sporangium); Aphanomyces (long, nematosporangium)

Life cycle of Saprolegnia (see figure 23-17; pg. 705 in Alexopoulos).

Sexual reproduction and hormone (steroids: antheridiol and oogoniol; production in Achlya (see previous handout, figure 10-5 in Biology of Fungi)

Peronosporales

Features: reniform zoospores; distinct sporangia; gametic meiosis; obligate parasites.

Pythiaceae: sporangiophores indistinct from mycelium; determinate in growth

Pythium: causes various kinds of wilts and damping off; has oval vesicles (see life cycle fig. 23-21, pg. 714 in textbook)

Phytophthora: zoospores in lemon-shaped sporangium that can function as a conidium if conditions are correct; sporangiophores are cross-shaped; causes late blight of potato and port orford root disease (see life cycle, fig. 23-25 on pg. 720 of textbook)

Albuginaceae (Albugo) (blister rust of crucifers): sporangia globose and in chains; subepidermal; sporangiophores undeterminate and unbranched

Peronosporaceae (downy mildews): branched sporangiophores; determinate.

See Fig. 23-26 on page 725 to distinguish between different genera. Life cycle of Plasmopara viticolas, causative agent of downy mildew of grapes (Fig. 23-26, pg. 727)

Leptomitales: sporangia resemble those of the Saprolegniales; see chart.

Aqualiderella has not cystochrome system; obligately fermentative; indicator of pollution.

Lagenidiales: holocarpic, endobiotic, parasite of algae. See chart.